PCR laboratory construction and installation plan
PCR laboratory construction and installation plan
The PCR laboratory is divided into four separate work areas: reagent storage and preparation area, sample preparation area, amplification reaction mixture preparation and amplification area, amplification product analysis area; in order to avoid cross-contamination, access to each work area It must be strictly followed in a single direction, that is, only from the test...
Text label: PCR laboratory construction, pharma machinery installation plan
PCR pharma machinery construction and installation plan
The PCR laboratory is divided into four separate work areas: reagent storage and preparation area, sample preparation area, amplification reaction mixture preparation and amplification area, amplification product analysis area; in order to avoid cross-contamination, access to each work area It must be carried out strictly in a single direction, that is, only from the reagent storage and preparation area → the sample preparation area → the amplification reaction mixture preparation and amplification area → the amplification product analysis area; the transfer of reagents and samples between the experimental areas should be carried out through the transfer In order to avoid the possibility of cross-contamination between each experimental area, the PCR pharma machinery should adopt the air organization form of full supply and full exhaust, and at the same time, the ratio of air supply and exhaust should be strictly controlled to ensure the pressure requirements of each experimental area.
Reagent storage and preparation area: The main operations in this experimental area are the preparation of storage reagents, the sub-packaging of reagents and the preparation of the main reaction mixture. The reagents and materials used for sample preparation should be directly transported to this area, and must not be Pass through other areas; reagent raw materials must be stored in this area, and the required storage reagents must be prepared in this area. Specimen preparation area: The main operations in this area are the preservation of clinical specimens, nucleic acid RNA, DNA extraction, storage, and cDNA synthesis when adding to the amplification reaction tube and measuring RNA; the pressure gradient requirements in this area are: relative to the adjacent area The area is under positive pressure to avoid aerosol contamination entering this area from the adjacent area; since contamination caused by aerosol may occur during the sample adding operation, unnecessary walking in this area should be avoided.
Amplification reaction mixture preparation and amplification area: The main operations in this area are DNA or cDNA amplification. The addition of the prepared DNA template and the synthesized cDNA and the preparation of the main reaction mixture into a reaction mixture can also be used. It is carried out in this area; it is negative pressure relative to the adjacent area to avoid aerosol leakage from this area; in order to avoid pollution caused by aerosol, unnecessary walking in this area should be minimized, and individual operations such as adding samples and so on should be carried out in the ultra-clean bench. Amplification product analysis area: The main operation in this area is the determination of amplified fragments. If a fully automatic closed analysis instrument is used for detection, this area may not be set up; this area is the main source of contamination of amplification products, so the pressure on this area is The requirements of the gradient are: a negative pressure relative to the adjacent area to avoid the diffusion of amplification products from this area to other areas. Zhongjing Global Purification can provide consulting, planning, design, construction, installation, transformation and other supporting services for PCR laboratories and microbiology laboratories.
Each experimental area of u200bu200bPCR must be set reasonably, and each experimental area must be clearly marked, such as eye-catching house numbers or different ground colors, so as to avoid confusion of equipment, items, reagents, etc. in different experimental areas; reasonable air conditioning and ventilation system For setting, try to use an air-conditioning system with full delivery and full exhaust; strict airflow pressure control ensures different pressure requirements in different experimental areas; during the experimental operation, the operator must wear gloves and change them frequently. In addition, it is used once during the operation. Sex caps are also an effective measure to prevent contamination; after the experiment is over, the area must be cleaned immediately. In addition to wiping and disinfecting surfaces with conventional disinfecting liquids or irradiating and disinfecting ultraviolet lamps, some experimental equipment should also be autoclaved. Treatment; strictly control the personnel entering and leaving the laboratory. Persons unrelated to the experiment are not allowed to enter and leave the laboratory at will. If conditions permit, independent passages and doors for entering and exiting the entire experimental area shall be set up; If the work clothes are of different colors, the work clothes in this area should not be taken to other areas when the staff leaves; unnecessary walking in the experimental area should be minimized to reduce the possibility of cross-contamination; the amplification product analysis area is the most important amplification area The source of product contamination, waste liquid cannot be dumped in the laboratory, must be immersed in disinfectant and then discarded in a place far away from the laboratory. Disposable materials such as used tips should also be immersed in disinfectant and disinfected and then disposed of uniformly.
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