PCR laboratory layout design
PCR laboratory layout design
PCR laboratory can design different partitions according to different operating platforms. In principle, the laboratory is divided into 4 separate working areas, namely reagent storage area, sample processing area, gene amplification area and amplification product analysis area. The areas must be completely separated, and there must be no air circulation; reagents...
Text label: PCR pharma machinery design and construction, PCR laboratory layout
PCR laboratory layout design
PCR laboratory can design different partitions according to different operating platforms. In principle, the pharma machinery is divided into 4 separate working areas, namely reagent storage area, sample processing area, gene amplification area and amplification product analysis area. The areas must be completely separated, and there should be no air circulation; reagents, samples, and experimental materials can only flow in one direction, from the reagent storage area → sample preparation area → gene amplification area → product analysis area, no reverse flow; The airflow should also flow from the first area to the rear area, and should not flow in the reverse direction. Each work area should be equipped with a buffer room, and an interlocking device should be installed between the work room and the buffer room; nucleic acid detection work areas with different functions should be separate and independent studios. There are obvious signs, each section cannot be directly connected, and each work room needs to install an item transfer window; each work area should be equipped with a UV lamp at the top, and the installation quantity is one 40W UV lamp per 20m2, and a movable UV lamp should be installed at the same time. .
The ideal layout of the laboratory is to have a dedicated corridor. The reagent storage area, sample preparation area, gene amplification area and product analysis area are arranged together in a standardized manner. Each area has a buffer area for changing work clothes and Work shoes are used; the working area of u200bu200bthe reagent storage area and the sample preparation area is relatively positive pressure, and the buffer area is relatively negative pressure; the working area of u200bu200bthe amplification area and the amplification product analysis area is relatively negative pressure, and the buffer area is relatively positive pressure. The pressure difference can be adjusted by the air supply and exhaust device. The doors in the buffer room leading to the inner laboratory and the corridor must be set diagonally and should not be directly opposite each other. Interlocking devices can be installed. When one door is opened, the other door must be closed. state, to prevent the situation that two doors are opened at the same time; each area must be clearly marked, and the work is carried out in a single flow direction of the reagent storage area, sample preparation area, gene amplification area, and product analysis area. If the real-time fluorescence quantitative PCR amplifier is used, the expansion area and the product analysis area can be combined, so that the entire PCR room is divided into three areas, namely reagent preparation area → sample preparation area → amplification analysis area; A fully automatic detection system with integrated analysis is added, and the latter two areas can be combined, so that the entire PCR room is divided into two areas, namely the reagent storage area and the gene detection area. Each area is completely independent, and an item transfer window is installed in the working area; each area needs to have an independent buffer room for changing work clothes and shoes, and ensure that the working area is always in a relative separation state; air supply and exhaust between areas The pipelines must be set up independently and must not be connected; ventilation devices should be installed in each working area and buffer room to adjust the air pressure. Zhongjing Global Purification can provide consulting, planning, design, construction, installation, transformation and other supporting services for PCR laboratories and clean laboratories.
A single flow of logistics, personnel and airflow should be formed between each area of u200bu200bthe laboratory to form a protection barrier with a single flow direction to avoid mutual interference between laboratories; in order to avoid cross-contamination between various experimental areas, it is advisable to use all-to-all The air organization form of the exhaust, and at the same time, the ratio of air supply and exhaust air should be strictly controlled to ensure the pressure requirements of each laboratory; the walls of the PCR laboratory, including the ceiling, should be firm and airtight, and all inner corners should be arc-shaped Line transition, the inner wall of the wall is smooth, non-adsorbing, corrosion-resistant, easy to clean and disinfect, and color steel plates are used for the wall and ceiling; the ground material should meet the requirements of no gap, no leakage, smoothness, and corrosion resistance; It can achieve the characteristics of easy cleaning and no dust accumulation; UV lamps are installed inside the transfer window; the experimental table should be waterproof, corrosion-resistant and heat-resistant, and epoxy resin table is generally used; the laboratory door is a purification door, and there should be a visual window with automatic Door closer, the door and the door cannot be opened relative to each other, and must be opened in dislocation; there is a reliable power supply and emergency lighting, and each room must be equipped with ultraviolet lights, and the installation height is between 1.8-2.1m.
PHARMA can also foster research that is more useful and influential in society at large.
SUZHOU PHARMA MACHINERY CO.,LTD. supports these goals with a corporate philosophy of adhering to the highest ethical conduct in all its business dealings, treatment of its employees, and social and environmental policies.
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